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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BBS10 Monoclonal antibody, PBS Only

BBS10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4C6A10

Cat no : 66679-1-PBS

Synonyms

Bardet Biedl syndrome 10, BBS10, C12orf58, FLJ23560

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BBS10 Monoclonal antibody (66679-1-Ig)

    WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BBS10 Monoclonal antibody (66679-1-Ig)

    WB analysis using 66679-1-Ig (same clone as 66679-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66679-1-Ig (BBS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BBS10 Monoclonal antibody (66679-1-Ig)

    IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BBS10 Monoclonal antibody (66679-1-Ig)

    IHC staining of human liver cancer using 66679-1-Ig (same clone as 66679-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66679-1-Ig (BBS10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66679-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66679-1-PBS targets BBS10 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BBS10 fusion protein Ag17960 相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 10
Calculated molecular weight 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC013795
Gene symbol BBS10
Gene ID (NCBI) 79738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).