Validation Data Gallery
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Tested Applications
| Positive WB detected in | NCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.
| Tested Reactivity | Human, pig, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MKS1 fusion protein Ag9504 相同性解析による交差性が予測される生物種 |
| Full Name | Meckel syndrome, type 1 |
| Calculated molecular weight | 559 aa, 65 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC010061 |
| Gene Symbol | MKS1 |
| Gene ID (NCBI) | 54903 |
| RRID | AB_2881881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NXB0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MKS1 antibody 66518-1-Ig | Download protocol |
| IHC protocol for MKS1 antibody 66518-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |