Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 9 publications below |
Product Information
11188-2-AP targets BBS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BBS2 fusion protein Ag1662 相同性解析による交差性が予測される生物種 |
| Full Name | Bardet-Biedl syndrome 2 |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC014140 |
| Gene Symbol | BBS2 |
| Gene ID (NCBI) | 583 |
| RRID | AB_2064971 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BBS2 antibody 11188-2-AP | Download protocol |
| IP protocol for BBS2 antibody 11188-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia. | ||
Cell Stem Cell Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression. | ||
J Cell Biol Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling. | ||
Brain Struct Funct A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice. | ||
Hum Mol Genet Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse. | ||
FASEB J Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis. |