BBS5 Monoclonal antibody
BBS5 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4G8E12
Cat no : 66136-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | mouse kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 66136-1-Ig is BBS5 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BBS5 fusion protein Ag6844 相同性解析による交差性が予測される生物種 |
| Full Name | Bardet-Biedl syndrome 5 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC044593 |
| Gene symbol | BBS5 |
| Gene ID (NCBI) | 129880 |
| RRID | AB_2881535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BBS5 antibody 66136-1-Ig | Download protocol |
| IHC protocol for BBS5 antibody 66136-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
