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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BBS9 Polyclonal antibody

BBS9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14460-1-AP

Synonyms

Protein PTHB1, Parathyroid hormone-responsive B1 gene protein, BBS 9, Bardet-Biedl syndrome 9 protein, Bardet Biedl syndrome 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BBS9 Polyclonal antibody (14460-1-AP)

    WB analysis using 14460-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using BBS9 Polyclonal antibody (14460-1-AP)

    WB analysis of mouse testis using 14460-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using BBS9 Polyclonal antibody (14460-1-AP)

    WB analysis of human heart using 14460-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14460-1-AP (BBS9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using BBS9 Polyclonal antibody (14460-1-AP)

    IP experiment of mouse testis using 14460-1-AP

    IP result of anti-BBS9 (IP:14460-1-AP, 4ug; Detection:14460-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using BBS9 Polyclonal antibody (14460-1-AP)

    IHC staining of human liver using 14460-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14460-1-AP (BBS9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using BBS9 Polyclonal antibody (14460-1-AP)

    IHC staining of human liver using 14460-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 14460-1-AP (BBS9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS9 Polyclonal antibody (14460-1-AP)

    IF Staining of hTERT-RPE1 cells using 14460-1-AP

    IF result (cytoplasm stain) of anti-BBS9 (14460-1-AP; 1:50) with hTERT-RPE1 cell by Dr. Moshe Kim.

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Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, human heart tissue, HeLa cells, Jurkat cells, mouse heart tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14460-1-AP targets BBS9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS9 fusion protein Ag5665 相同性解析による交差性が予測される生物種
Full Name Bardet-Biedl syndrome 9
Calculated molecular weight 99 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032715
Gene symbol BBS9
Gene ID (NCBI) 27241
RRIDAB_10598001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BBS9 antibody 14460-1-APDownload protocol
IHC protocol for BBS9 antibody 14460-1-APDownload protocol
IP protocol for BBS9 antibody 14460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
View Article
humanWB

EMBO Rep

IFT proteins interact with HSET to promote supernumerary centrosome clustering in mitosis.

Authors - Benjamin Vitre
View Article
mouseWB

Invest Ophthalmol Vis Sci

Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis.

Authors - Satyabrata Sinha
View Article