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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCAM Polyclonal antibody

BCAM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28222-1-AP

Synonyms

LU, F8/G253 antigen, CD239, B-CAM cell surface glycoprotein, Basal cell adhesion molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAM Polyclonal antibody (28222-1-AP)

    WB analysis using 28222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28222-1-AP (BCAM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA431 cells, HT-29 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28222-1-AP targets BCAM in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAM fusion protein Ag28181 相同性解析による交差性が予測される生物種
Full Name basal cell adhesion molecule (Lutheran blood group)
Calculated molecular weight 67 kDa
Observed molecular weight67 kDa, 78-85 kDa
GenBank accession numberBC050450
Gene symbol BCAM
Gene ID (NCBI) 4059
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Basal cell adhesion molecule (Lu/BCAM) is a membrane-bound glycoprotein of the immunoglobulin superfamily (IgSF), functioning as a receptor for the extracellular matrix protein, laminin. BCAM (Lutheran/basal cell-adhesion molecule, a glycoprotein) belongs to the immunoglobulin superfamily which contains both Lu blood group and BCAM tumor-associated antigens. Proteomics analysis suggests that BCAM might be a potential biomarker for pancreatic cancer (PMID: 19199705). BCAM, involved in cell adhesion and migration, can also promote tumor metastasis and has been elucidated to play a functional role in the metastasis of thyroid cancer and gastric cancer (PMID: 10728810, 35941663). De-glycosylated BCAM has a preidicted molecular weight of 67 kDa. Two BCAM glycoprotein (gp) isoforms are present on human RBCs, Lu (85 kDa) and Lu(v13) (78 kDa); they result from the alternative splicing of intron 13 and differ by the lengths of their cytoplasmic domains (PMID: 23160466).

Protocols

Product Specific Protocols
WB protocol for BCAM antibody 28222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols