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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCAN Recombinant antibody

BCAN Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230126D6

Cat no : 82956-1-RR

Synonyms

Brevican, CSPG7, Brevican core protein, Brain-enriched hyaluronan-binding protein, BEHAB

Validation Data Gallery

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  • Flow cytometry (FC) experiment of SH-SY5Y cells using BCAN Recombinant antibody (82956-1-RR)

    FC experiment of SH-SY5Y using 82956-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Anti-Human BCAN (82956-1-RR, Clone:230126D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82956-1-RR targets BCAN in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCAN fusion protein Ag13596 相同性解析による交差性が予測される生物種
Full Name brevican
Calculated molecular weight 99 kDa
GenBank accession numberBC010571
Gene symbol BCAN
Gene ID (NCBI) 63827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Brevican is a brain-specific proteoglycan belonging to the aggrecan family that share a number of structural features. Brevican contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. Expression of brevican is highly specific in the brain and increases as the brain develops. It may play a role in maintaining the extracellular environment of mature brain as a major constituent of the adult brain extracellular matrix (PMID:9117262). Brevican core protein exists as a 145 kDa full-length form and a 80 kDa N terminally truncated form (PMID: 8144512). This antibody can recognize the 72 kDa isoform of target.

Protocols

Product Specific Protocols
FC protocol for BCAN antibody 82956-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols