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KD/KO Validated

BAP31 Polyclonal antibody

Name: BAP31 Polyclonal antibody
Brand: Proteintech
SKU: 11200-1-AP
Rating: 5.00 (1 reviews)

BAP31 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11200-1-AP

Synonyms

BCAP31, CDM, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11200-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11200-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11200-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 11200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11200-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 11200-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11200-1-AP (BAP31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 11200-1-AP
IP result of anti-BAP31 (IP:11200-1-AP, 3ug; Detection:11200-1-AP 1:1000) with A431 cells lysate 3300ug.
IHC staining of human breast cancer using 11200-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11200-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11200-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11200-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11200-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11200-1-AP (BAP31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11200-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (11200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), phalloidine stains F-actin (red).

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Cat No. 11200-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, human placenta tissue, HEK-293 cells, HeLa cells, mouse placenta tissue, MCF-7 cells, THP-1 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tisssue
Positive IP detected in	A431 cells
Positive IHC detected in	human breast cancer tissue, human colon tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 16 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

11200-1-AP targets BAP31 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BAP31 fusion protein Ag1668 相同性解析による交差性が予測される生物種
Full Name	B-cell receptor-associated protein 31
Calculated molecular weight	28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC014323
Gene symbol	BAP31
Gene ID (NCBI)	10134
RRID	AB_2065142
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Protocols

Product Specific Protocols
WB protocol for BAP31 antibody 11200-1-AP	Download protocol
IHC protocol for BAP31 antibody 11200-1-AP	Download protocol
IF protocol for BAP31 antibody 11200-1-AP	Download protocol
IP protocol for BAP31 antibody 11200-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nat Biotechnol Efficient proximity labeling in living cells and organisms with TurboID. Authors - Tess C Branon View Article
human	WB	Nat Methods Directed evolution of APEX2 for electron microscopy and proximity labeling. Authors - Stephanie S Lam View Article
human	WB	Nat Commun HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Authors - Ulrike Zinnall View Article
human	WB	EMBO J FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions. Authors - Wenxian Wu View Article
human	WB,IP	EMBO J Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction. Authors - Iwasawa Ryota R KD Validated View Article
human		EMBO Rep Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling Authors - Misha Kalarikkal View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Boyan (Verified Customer) (11-02-2020)

This antibody labeled the ER area clearly

Applications: Immunofluorescence

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAP31 Polyclonal antibody

BAP31 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11200-1-AP

WB analysis of HEK-293 using 11200-1-AP

WB analysis of human placenta using 11200-1-AP

WB analysis of A431 using 11200-1-AP

WB analysis of HeLa using 11200-1-AP

WB analysis of mouse placenta using 11200-1-AP

IP experiment of A431 using 11200-1-AP

IHC staining of human breast cancer using 11200-1-AP

IHC staining of human breast cancer using 11200-1-AP

IHC staining of human colon using 11200-1-AP

IHC staining of human colon cancer using 11200-1-AP

IHC staining of human colon cancer using 11200-1-AP

IF Staining of HeLa using 11200-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Efficient proximity labeling in living cells and organisms with TurboID.

Directed evolution of APEX2 for electron microscopy and proximity labeling.

HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction.

Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling

Reviews

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