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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAP31 Recombinant antibody

BAP31 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241978D10

Cat no : 84584-5-RR

Synonyms

BCAP31, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, 6C6 AG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAP31 Recombinant antibody (84584-5-RR)

    WB analysis using 84584-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84584-5-RR (BAP31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAP31 Recombinant antibody (84584-5-RR)

    IF Staining of HeLa using 84584-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (84584-5-RR, Clone: 241978D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, THP-1 cells, mouse liver tissue, rat liver tisssue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84584-5-RR targets BAP31 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BAP31 fusion protein Ag1668 相同性解析による交差性が予測される生物種
Full Name B-cell receptor-associated protein 31
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC014323
Gene symbol BAP31
Gene ID (NCBI) 10134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAP31,aiso know as BCAP31, is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein, and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Protocols

Product Specific Protocols
WB protocol for BAP31 antibody 84584-5-RRDownload protocol
IF protocol for BAP31 antibody 84584-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols