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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BAP31 Recombinant antibody

BAP31 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241978D10

Cat no : 84584-5-RR

Synonyms

BCAP31, BCR-associated protein 31, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, 6C6 AG

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAP31 Recombinant antibody (84584-5-RR)

    WB analysis using 84584-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84584-5-RR (BAP31 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BAP31 Recombinant antibody (84584-5-RR)

    IHC staining of human colon cancer using 84584-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BAP31 Recombinant antibody (84584-5-RR)

    IHC staining of human colon cancer using 84584-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using BAP31 Recombinant antibody (84584-5-RR)

    IHC staining of human placenta using 84584-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using BAP31 Recombinant antibody (84584-5-RR)

    IHC staining of human placenta using 84584-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84584-5-RR (BAP31 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAP31 Recombinant antibody (84584-5-RR)

    IF Staining of HeLa using 84584-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BAP31 antibody (84584-5-RR, Clone: 241978D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using BAP31 Recombinant antibody (84584-5-RR)

    FC experiment of HepG2 using 84584-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug BAP31 Recombinant antibody (84584-5-RR, Clone:241978D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of PC-3 cells using BAP31 Recombinant antibody (84584-5-RR)

    FC experiment of PC-3 using 84584-5-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug BAP31 Recombinant antibody (84584-5-RR, Clone:241978D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84584-5-RR

    Affinity and Kinetic Characterization of 84584-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84584-5-RR against Human BAP31 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, MCF-7 cells, THP-1 cells, mouse liver tissue, rat liver tisssue
Positive IHC detected inhuman colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84584-5-RR targets BAP31 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BAP31 fusion protein Ag1668 相同性解析による交差性が予測される生物種
Full Name B-cell receptor-associated protein 31
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC014323
Gene symbol BAP31
Gene ID (NCBI) 10134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAP31, also known as BCAP31, is a chaperone protein abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded proteins, and their targeting to the ER-associated-degradation (ERAD). CDIP1 and BAP31 interact upon ER stress to regulate the mitochondrial apoptosis pathway(PMID: 24139803). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.

Protocols

Product Specific Protocols
WB protocol for BAP31 antibody 84584-5-RRDownload protocol
IHC protocol for BAP31 antibody 84584-5-RRDownload protocol
IF protocol for BAP31 antibody 84584-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols