Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10414-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10414-1-AP
WB result of BCAS2 antibody (10414-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCAS2 transfected HeLa cells.
WB analysis of human brain using 10414-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10414-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 10414-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10414-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 10414-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10414-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10414-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of hESC using 10414-1-AP
hESC cells were subjected to SDS PAGE followed by western blot with 10414-1-AP (BCAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10414-1-AP
IP result of anti-BCAS2 (IP:10414-1-AP, 3ug; Detection:10414-1-AP 1:1000) with mouse brain tissue lysate 6500ug.
IHC staining of human prostate cancer using 10414-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10414-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 10414-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 10414-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10414-1-AP (BCAS2 antibody) at dilution of 1:100 (under 25x lens).
IHC staining of human prostate cancer using 10414-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10414-1-AP (BCAS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10414-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCAS2 antibody (10414-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of MCF-7 using 10414-1-AP
Immunofluorescent analysis of MCF-7 cells using 10414-1-AP (BCAS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

BCAS2 Polyclonal antibody

BCAS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10414-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

Spliceosome-associated protein SPF 27, Pre-mRNA-splicing factor SPF27, Pre mRNA splicing factor SPF27, DAM1, Breast carcinoma-amplified sequence 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, mouse liver tissue, hESC cells, human brain tissue, K-562 cells, COS-7 cells, SH-SY5Y cells, PC-12 cells, C6 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human prostate cancer tissue, human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 2 publications below
RIP	See 1 publications below

Product Information

10414-1-AP targets BCAS2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BCAS2 fusion protein Ag0669 相同性解析による交差性が予測される生物種
Full Name	breast carcinoma amplified sequence 2
Calculated molecular weight	26 kDa
Observed molecular weight	26-32 kDa
GenBank accession number	BC005285
Gene Symbol	BCAS2
Gene ID (NCBI)	10286
RRID	AB_2063400
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75934
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BCAS2 associates with the spliceosome and implicates in mRNA splicing

Protocols

Product Specific Protocols
WB protocol for BCAS2 antibody 10414-1-AP	Download protocol
IHC protocol for BCAS2 antibody 10414-1-AP	Download protocol
IF protocol for BCAS2 antibody 10414-1-AP	Download protocol
IP protocol for BCAS2 antibody 10414-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Authors - Wenbo Liu View Article
	WB	Int J Biochem Cell Biol BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination. Authors - Shengjie Liao View Article
mouse	WB,IF	Stem Cell Res Ther Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin. Authors - Hsin-Hsiung Chen KD Validated View Article
human	IHC, WB	Br J Cancer BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability. Authors - P-C Kuo KD Validated View Article
mouse	WB,IF	FASEB J BCAS2 is involved in alternative splicing and mouse oocyte development. Authors - Jiaqi Zhang KO Validated View Article
mouse	WB,RIP	Endocrinology BCAS2 participates in insulin synthesis and secretion via mRNA alternative splicing in mice Authors - Xuexue Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10414-1-AP

WB analysis of HeLa using 10414-1-AP

WB analysis of human brain using 10414-1-AP

WB analysis of K-562 using 10414-1-AP

WB analysis of COS-7 using 10414-1-AP

WB analysis of HeLa using 10414-1-AP

WB analysis of SH-SY5Y using 10414-1-AP

WB analysis of mouse brain using 10414-1-AP

WB analysis of mouse liver using 10414-1-AP

WB analysis of hESC using 10414-1-AP

IP experiment of mouse brain using 10414-1-AP

IHC staining of human prostate cancer using 10414-1-AP

IHC staining of human breast cancer using 10414-1-AP

IHC staining of human breast cancer using 10414-1-AP

IHC staining of human colon cancer using 10414-1-AP

IHC staining of human prostate cancer using 10414-1-AP

IF Staining of HeLa using 10414-1-AP

IF Staining of MCF-7 using 10414-1-AP

BCAS2 Polyclonal antibody

BCAS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis.

BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination.

Breast carcinoma-amplified sequence 2 regulates adult neurogenesis via β-catenin.

BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability.

BCAS2 is involved in alternative splicing and mouse oocyte development.

BCAS2 participates in insulin synthesis and secretion via mRNA alternative splicing in mice