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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCAS2 Monoclonal antibody, PBS Only

BCAS2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A12G1

Cat no : 68003-1-PBS

Synonyms

BCAS2, DAM1, Pre mRNA splicing factor SPF27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis using 68003-1-Ig (same clone as 68003-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis of LNCaP using 68003-1-Ig (same clone as 68003-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis of HeLa using 68003-1-Ig (same clone as 68003-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using BCAS2 Monoclonal antibody (68003-1-Ig)

    WB analysis of HEK-293 using 68003-1-Ig (same clone as 68003-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68003-1-Ig (BCAS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68003-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68003-1-PBS targets BCAS2 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCAS2 fusion protein Ag21309 相同性解析による交差性が予測される生物種
Full Name breast carcinoma amplified sequence 2
Calculated molecular weight 26 kDa
Observed molecular weight28 kDa
GenBank accession numberBC005285
Gene symbol BCAS2
Gene ID (NCBI) 10286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Breast carcinoma amplified sequence 2 (BCAS2) is preferentially known as pre-mRNA splicing factor SPF27 and was originally characterized as an up-regulated gene by amplification in human breast cancer cells. BCAS2 has been shown to be involved in DNA damage repair through the replication protein A (RPA) complex.