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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAS3 Monoclonal antibody (67085-1-Ig)

    WB analysis using 67085-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BCAS3 Monoclonal antibody (67085-1-Ig)

    WB analysis of K-562 using 67085-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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BCAS3 Monoclonal antibody

BCAS3 Monoclonal Antibody for WB, ELISA
Cat No. 67085-1-Ig
Clone No.1G7B1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Synonyms

BCAS3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, Protein Maab1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU2OS cells, K-562 cells, Jurkat cells, A431 cells, HEK-293 cells, A549 cells, LNCaP cells, pig brain tissue, rat brain tissue, mouse rat tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67085-1-Ig targets BCAS3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCAS3 fusion protein Ag28545 相同性解析による交差性が予測される生物種
Full Name breast carcinoma amplified sequence 3
Calculated molecular weight 99 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC001250
Gene Symbol BCAS3
Gene ID (NCBI) 54828
RRIDAB_2882393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H6U6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.

Protocols

Product Specific Protocols
WB protocol for BCAS3 antibody 67085-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols