BCAS3 Monoclonal antibody
BCAS3 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G7B1
Cat no : 67085-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U2OS cells, K-562 cells, Jurkat cells, A431 cells, HEK-293 cells, A549 cells, LNCaP cells, pig brain tissue, rat brain tissue, mouse rat tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:20000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67085-1-Ig targets BCAS3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | BCAS3 fusion protein Ag28545 相同性解析による交差性が予測される生物種 |
Full Name | breast carcinoma amplified sequence 3 |
Calculated molecular weight | 99 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC001250 |
Gene symbol | BCAS3 |
Gene ID (NCBI) | 54828 |
RRID | AB_2882393 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.
Protocols
Product Specific Protocols | |
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WB protocol for BCAS3 antibody 67085-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |