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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCAT1 Polyclonal antibody, PBS Only

BCAT1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28622-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAT1 Polyclonal antibody (28622-1-AP)

    WB analysis using 28622-1-AP (same clone as 28622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human liver cancer using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human lung cancer using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human gliomas using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28622-1-PBS targets BCAT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCAT1 fusion protein Ag28998 相同性解析による交差性が予測される生物種
Full Name branched chain aminotransferase 1, cytosolic
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC033864
Gene symbol BCAT1
Gene ID (NCBI) 586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).