• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCAT1 Monoclonal antibody

BCAT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G3A10

Cat no : 67084-1-Ig

Synonyms

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Ramos cells using BCAT1 Monoclonal antibody (67084-1-Ig)

    WB analysis of Ramos using 67084-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using BCAT1 Monoclonal antibody (67084-1-Ig)

    WB analysis of pig brain using 67084-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using BCAT1 Monoclonal antibody (67084-1-Ig)

    WB analysis of Daudi using 67084-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inRamos cells, Daudi cells, pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67084-1-Ig targets BCAT1 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCAT1 fusion protein Ag28328 相同性解析による交差性が予測される生物種
Full Name branched chain aminotransferase 1, cytosolic
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC033864
Gene symbol BCAT1
Gene ID (NCBI) 586
RRIDAB_2882392
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).

Protocols

Product Specific Protocols
WB protocol for BCAT1 antibody 67084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols