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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCAT2 Monoclonal antibody (68586-1-Ig)

    WB analysis using 68586-1-Ig (same clone as 68586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using BCAT2 Monoclonal antibody (68586-1-Ig)

    IF Staining of A431 using 68586-1-Ig (same clone as 68586-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using BCAT2 antibody (68586-1-Ig, Clone: 1A5A5 ) at dilution of 1:1130 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50268-2

    Cytometric bead array standard curve of MP50268-2

    Cytometric bead array standard curve of MP50268-2, BCAT2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68586-1-PBS. Detection antibody: 68586-4-PBS. Standard:Ag9595. Range: 0.781-100 ng/mL.

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BCAT2 Monoclonal antibody, PBS Only (Capture)

BCAT2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68586-1-PBS
Clone No.1A5A5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68586-1-PBS targets BCAT2 as part of a matched antibody pair:

MP50268-2: 68586-1-PBS capture and 68586-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BCAT2 fusion protein Ag9595 相同性解析による交差性が予測される生物種
Full Name branched chain aminotransferase 2, mitochondrial
Calculated molecular weight 44 kDa
Observed molecular weight38 kDa
GenBank accession numberBC001900
Gene Symbol BCAT2
Gene ID (NCBI) 587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15382
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).