BCHE Polyclonal antibody

BCHE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23854-1-AP

Synonyms

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, butyrylcholinesterase, CHE1, Choline esterase II, Cholinesterase, E1, Pseudocholinesterase


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23854-1-AP targets BCHE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCHE fusion protein Ag20888 相同性解析による交差性が予測される生物種
Full Name butyrylcholinesterase
Calculated molecular weight 602 aa, 68 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC008396
Gene symbol BCHE
Gene ID (NCBI) 590
RRIDAB_2879341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCHE(butyrylcholinesterase) is an enzyme expressed in most human tissues. It may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease(PMID:11848688). BCHE is also named as CHE1 and belongs to the type-B carboxylesterase/lipase family. This is a glycoprotein with the molecular weight from 68 kDa to 90 kDa.

Protocols

Product Specific Protocols
WB protocol for BCHE antibody 23854-1-APDownload protocol
IHC protocol for BCHE antibody 23854-1-APDownload protocol
IF protocol for BCHE antibody 23854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Biomed Eng

Genome-edited skin epidermal stem cells protect mice from cocaine-seeking behaviour and cocaine overdose.

Authors - Yuanyuan Li
goatWB,IF

Cells

Improving the Efficiency of Precise Genome Editing with CRISPR/Cas9 to Generate Goats Overexpressing Human Butyrylcholinesterase

Authors - Jia-Hao Wang
humanWB,IHC

Front Immunol

Progressions of the correlation between lipid metabolism and immune infiltration characteristics in gastric cancer and identification of BCHE as a potential biomarker

Authors - Shibo Wang