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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using BCKDK Polyclonal antibody (15718-1-AP)

    WB analysis of mouse pancreas using 15718-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BCKDK Polyclonal antibody (15718-1-AP)

    WB analysis using 15718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using BCKDK Polyclonal antibody (15718-1-AP)

    IHC staining of mouse pancreas using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using BCKDK Polyclonal antibody (15718-1-AP)

    IHC staining of mouse pancreas using 15718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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BCKDK Polyclonal antibody

BCKDK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15718-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

BCKDK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15718-1-AP targets BCKDK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCKDK fusion protein Ag8395 相同性解析による交差性が予測される生物種
Full Name branched chain ketoacid dehydrogenase kinase
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC007363
Gene Symbol BCKDK
Gene ID (NCBI) 10295
RRIDAB_2878174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BCKDK antibody 15718-1-APDownload protocol
IHC protocol for BCKDK antibody 15718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.

Authors - Yang Yang
View Article
mouseWB

Biochim Biophys Acta Mol Basis Dis

Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury

Authors - Xiao Han
View Article