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  • Featured Product
  • KD/KO Validated

BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 50180-1-AP

Synonyms

CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using BCL10 Polyclonal antibody (50180-1-AP)

    WB analysis of K-562 using 50180-1-AP

    WB result of BCL10 antibody (50180-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL10 transfected K-562 cells.

  • Western Blot (WB) analysis of various lysates using BCL10 Polyclonal antibody (50180-1-AP)

    WB analysis using 50180-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 50180-1-AP (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BCL10 Polyclonal antibody (50180-1-AP)

    IHC staining of human lung cancer using 50180-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BCL10 Polyclonal antibody (50180-1-AP)

    IHC staining of human lung cancer using 50180-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BCL10 Polyclonal antibody (50180-1-AP)

    IHC staining of human colon cancer using 50180-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BCL10 Polyclonal antibody (50180-1-AP)

    IHC staining of human colon cancer using 50180-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 50180-1-AP (BCL10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BCL10 Polyclonal antibody (50180-1-AP)

    IF Staining of A549 using 50180-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BCL10 antibody (50180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, K-562 cells, Jurkat cells, Raji cells, Ramos cells, mouse ovary tissue, rat ovary tissue
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

50180-1-AP targets BCL10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL10 fusion protein Ag0037 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 10
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 26-32 kDa
GenBank accession numberBC053617
Gene symbol BCL10
Gene ID (NCBI) 8915
RRIDAB_2243501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

Protocols

Product Specific Protocols
WB protocol for BCL10 antibody 50180-1-APDownload protocol
IHC protocol for BCL10 antibody 50180-1-APDownload protocol
IF protocol for BCL10 antibody 50180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

The IRAK-1-BCL10-MALT1-TRAF6-TAK1 cascade mediates signaling to NF-kappaB from Toll-like receptor 4.

Authors - Dong Wei W
View Article
mouseWB

J Biol Chem

BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2.

Authors - Liu Yingle Y
  • KD Validated
View Article
humanWB

Cell Signal

The paracaspase MALT1 is a downstream target of Smad3 and potentiates the crosstalk between TGF-β and NF-kB signaling pathways in cancer cells

Authors - Fatma Aybuke Mazi
View Article