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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL10 Monoclonal antibody, PBS Only

BCL10 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E8G5

Cat no : 66556-1-PBS

Synonyms

B cell CLL/lymphoma 10, B cell lymphoma/leukemia 10, BCL10, c E10, CARD like apoptotic protein, CARMEN, cCARMEN, Cellular E10, Cellular homolog of vCARMEN, CIPER, CLAP, hCLAP, mE10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL10 Monoclonal antibody (66556-1-Ig)

    WB analysis using 66556-1-Ig (same clone as 66556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using BCL10 Monoclonal antibody (66556-1-Ig)

    WB analysis of Raji using 66556-1-Ig (same clone as 66556-1-PBS)

    Raji, K-562, and HL-60 cells were subjected to SDS PAGE followed by western blot with 66556-1-Ig (BCL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human tonsillitis using 66556-1-Ig (same clone as 66556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human tonsillitis using 66556-1-Ig (same clone as 66556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human lymphoma using 66556-1-Ig (same clone as 66556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL10 Monoclonal antibody (66556-1-Ig)

    IHC staining of human lymphoma using 66556-1-Ig (same clone as 66556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66556-1-Ig (BCL10 antibody) at dilution of 1:700 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCL10 Monoclonal antibody (66556-1-Ig)

    IF Staining of HepG2 using 66556-1-Ig (same clone as 66556-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BCL10 antibody (66556-1-Ig, Clone: 2E8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66556-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66556-1-PBS targets BCL10 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCL10 fusion protein Ag12162 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 10
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC053617
Gene symbol BCL10
Gene ID (NCBI) 8915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.