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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCL11A Recombinant antibody (84930-3-RR)

    WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BCL11A Recombinant antibody (84930-3-RR)

    WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01680-2

    Sandwich ELISA standard curve of MP01680-2

    Sandwich ELISA standard curve of MP01680-2, Human BCL11A Recombinant Matched Antibody Pair - PBS only. 84930-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2187. 84930-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01680-2

    Sample test of MP01680-2

    The mean BCL11A concentration was determined to be 16.52 ng/mL in U-937 cell extract based on a 2.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP01680-2

    Cytometric bead array standard curve of MP01680-2

    Cytometric bead array standard curve of MP01680-2, BCL11A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84930-3-PBS. Detection antibody: 84930-4-PBS. Standard: Ag2187. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84930-3-RR

    Affinity and Kinetic Characterization of 84930-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84930-3-RR against Human BCL11A were performed. The affinity constant is 1.38 nM.

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BCL11A Recombinant antibody, PBS Only (Capture)

BCL11A Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84930-3-PBS
Clone No.242481F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

COUP-TF-interacting protein 1, BCL-11A, B-cell lymphoma/leukemia 11A, B-cell CLL/lymphoma 11A, B cell lymphoma/leukaemia 11A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84930-3-PBS targets BCL11A as part of a matched antibody pair:

MP01680-2: 84930-3-PBS capture and 84930-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL11A fusion protein Ag2187 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 11A (zinc finger protein)
Calculated molecular weight 835 aa, 91 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC021098
Gene Symbol BCL11A
Gene ID (NCBI) 53335
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H165
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.