Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of A431 using 12789-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat cells using 12789-1-AP
WB result of BCL2 (12789-1-AP, 1:1000) with si-Control and si-BCL2 transfected Jurkat cells.
WB analysis using 12789-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12789-1-AP (human BCL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12789-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12789-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 12789-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 12789-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 12789-1-AP (BCL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HL-60 using 12789-1-AP
IP result of anti-human BCL2 (IP:12789-1-AP, 4ug; Detection:12789-1-AP 1:1000) with HL-60 cells lysate 4000 ug.
IP experiment of HepG2 using 12789-1-AP
IP result of anti-human BCL2 (IP:12789-1-AP, 3ug; Detection:12789-1-AP 1:500) with HepG2 cells lysate 4500ug.
IP experiment of MCF-7 using 12789-1-AP
IP result of anti-human BCL2 (IP:12789-1-AP, 3ug; Detection:12789-1-AP 1:1000) with MCF-7 cells lysate 2500ug.
IHC staining of human tonsillitis using 12789-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 12789-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 12789-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (human BCL2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 12789-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12789-1-AP (human BCL2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12789-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12789-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12789-1-AP (BCL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Jurkat using 12789-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using human BCL2 antibody (12789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HeLa using 12789-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.8 ug human BCL2 Polyclonal antibody (12789-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

human BCL2 Polyclonal antibody

human BCL2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12789-1-AP

Publications(2531)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (7)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA

Synonyms

BCL-2, BCL2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2531)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HepG2 cells, Jurkat cells, HEK-293 cells, HL-60 cells, U-251 cells, U-937 cells, MCF-7 cells, U2OS cells
Positive IP detected in	HL-60 cells, HepG2 cells, MCF-7 cells
Positive IHC detected in	human tonsillitis tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Jurkat cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2442 publications below
IHC	See 116 publications below
IF	See 47 publications below
IP	See 2 publications below
FC	See 1 publications below
CoIP	See 6 publications below
ChIP	See 1 publications below

Product Information

12789-1-AP targets human BCL2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rabbit, chicken, zebrafish, hamster, sheep, goat, fish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	human BCL2 fusion protein Ag3508 相同性解析による交差性が予測される生物種
Full Name	B-cell CLL/lymphoma 2
Calculated molecular weight	26 kDa
Observed molecular weight	25-30 kDa
GenBank accession number	BC027258
Gene Symbol	BCL2
Gene ID (NCBI)	596
RRID	AB_2227948
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10415
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

1. What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2. FAQs for Bcl-2

a. How to measure Bcl-2 and Bax apoptotic markers by western blotting

Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

b. What band size should I expect in western blotting for Bcl-2?

The molecular weight of Bcl-2 is 26 kDa.

Protocols

Product Specific Protocols
WB protocol for human BCL2 antibody 12789-1-AP	Download protocol
IHC protocol for human BCL2 antibody 12789-1-AP	Download protocol
IF protocol for human BCL2 antibody 12789-1-AP	Download protocol
IP protocol for human BCL2 antibody 12789-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy Authors - Yang Meng View Article
	WB,CoIP	Cell Metab Exercise-activated hepatic autophagy via the FN1-α5β1 integrin pathway drives metabolic benefits of exercise Authors - Kenta Kuramoto View Article
pig	WB	Cell Stem Cell Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation Authors - Jiaowei Wang View Article
human	WB	ACS Nano Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer. Authors - Danielle M Valcourt View Article
	WB	ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy Authors - Qiqi Zhang View Article
		ACS Nano Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway Authors - Xirui Xin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 12789-1-AP

WB analysis of Jurkat cells using 12789-1-AP

WB analysis using 12789-1-AP

WB analysis of HepG2 using 12789-1-AP

WB analysis of HEK-293 using 12789-1-AP

WB analysis using 12789-1-AP

WB analysis of U-251 using 12789-1-AP

IP experiment of HL-60 using 12789-1-AP

IP experiment of HepG2 using 12789-1-AP

IP experiment of MCF-7 using 12789-1-AP

IHC staining of human tonsillitis using 12789-1-AP

IHC staining of human tonsillitis using 12789-1-AP

IHC staining of human tonsillitis using 12789-1-AP

IHC staining of human tonsillitis using 12789-1-AP

IHC staining of human lymphoma using 12789-1-AP

IHC staining of human lymphoma using 12789-1-AP

IF Staining of Jurkat using 12789-1-AP

FC experiment of HeLa using 12789-1-AP

human BCL2 Polyclonal antibody

human BCL2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Exercise-activated hepatic autophagy via the FN1-α5β1 integrin pathway drives metabolic benefits of exercise

Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementation

Nanoparticle-Mediated Co-Delivery of Notch-1 Antibodies and ABT-737 as a Potent Treatment Strategy for Triple-Negative Breast Cancer.

Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway