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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL2L12 Polyclonal antibody

BCL2L12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21969-1-AP

Synonyms

BCL2L12, BPR

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using BCL2L12 Polyclonal antibody (21969-1-AP)

    WB analysis of PC-3 using 21969-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21969-1-AP (BCL2L12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BCL2L12 Polyclonal antibody (21969-1-AP)

    IHC staining of human breast cancer using 21969-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21969-1-AP (BCL2L12 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BCL2L12 Polyclonal antibody (21969-1-AP)

    IHC staining of human breast cancer using 21969-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21969-1-AP (BCL2L12 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using BCL2L12 Polyclonal antibody (21969-1-AP)

    IF Staining of PC-3 using 21969-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21969-1-AP (BCL2L12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21969-1-AP targets BCL2L12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2L12 fusion protein Ag16856 相同性解析による交差性が予測される生物種
Full Name BCL2-like 12 (proline rich)
Calculated molecular weight 334 aa, 37 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC104005
Gene symbol BCL2L12
Gene ID (NCBI) 83596
RRIDAB_2878956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BCL2L12 antibody 21969-1-APDownload protocol
IHC protocol for BCL2L12 antibody 21969-1-APDownload protocol
IF protocol for BCL2L12 antibody 21969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols