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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL2L15 Polyclonal antibody

BCL2L15 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23975-1-AP

Synonyms

C1orf178, Bfk, Bcl-2-like protein 15, Bcl2-L-15, Bcl-2 family kin

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human colon using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human appendicitis using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human colon cancer using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human appendicitis using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    IF Staining of HEK-293 using 23975-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    IF Staining of HEK-293 using 23975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman colon tissue, human appendicitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

23975-1-AP targets BCL2L15 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2L15 fusion protein Ag21155 相同性解析による交差性が予測される生物種
Full Name BCL2-like 15
Calculated molecular weight 163 aa, 18 kDa
GenBank accession numberBC127719
Gene symbol BCL2L15
Gene ID (NCBI) 440603
RRIDAB_3085718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B-cell lymphoma 2 (Bcl-2)-like protein 15 (BCL2L15), also known as BCL2 family kin (BFK), belongs to the BCL2 protein family, and appears to induce weak apoptosis and antagonize the pro-survival function of Bcl2 (PMID:32708974). In addition, studies reported BCL2L15 expression in the stomach, ovaries, bone marrow, spleen, and uterus (PMID:12700646).

Protocols

Product Specific Protocols
IHC protocol for BCL2L15 antibody 23975-1-APDownload protocol
IF protocol for BCL2L15 antibody 23975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Transl Med

CTPAD: an interactive web application for comprehensive transcriptomic profiling in allergic diseases

Authors - Suizi Zhou
View Article