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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL2L15 Polyclonal antibody

BCL2L15 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23975-1-AP

Synonyms

C1orf178, Bfk, Bcl-2-like protein 15, Bcl2-L-15, Bcl-2 family kin

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human colon using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human appendicitis using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human colon cancer using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using BCL2L15 Polyclonal antibody (23975-1-AP)

    IHC staining of human appendicitis using 23975-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    IF Staining of HEK-293 using 23975-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BCL2L15 Polyclonal antibody (23975-1-AP)

    IF Staining of HEK-293 using 23975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman colon tissue, human appendicitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23975-1-AP targets BCL2L15 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2L15 fusion protein Ag21155 相同性解析による交差性が予測される生物種
Full Name BCL2-like 15
Calculated molecular weight 163 aa, 18 kDa
GenBank accession numberBC127719
Gene symbol BCL2L15
Gene ID (NCBI) 440603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B-cell lymphoma 2 (Bcl-2)-like protein 15 (BCL2L15), also known as BCL2 family kin (BFK), belongs to the BCL2 protein family, and appears to induce weak apoptosis and antagonize the pro-survival function of Bcl2 (PMID:32708974). In addition, studies reported BCL2L15 expression in the stomach, ovaries, bone marrow, spleen, and uterus (PMID:12700646).

Protocols

Product Specific Protocols
IHC protocol for BCL2L15 antibody 23975-1-APDownload protocol
IF protocol for BCL2L15 antibody 23975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols