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  • Featured Product
  • KD/KO Validated

BCL3 Polyclonal antibody

BCL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 23959-1-AP

Synonyms

B cell CLL/lymphoma 3, B cell lymphoma 3 protein, BCL3, D19S37, Proto oncogene BCL3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BCL3 Polyclonal antibody (23959-1-AP)

    WB analysis of HeLa using 23959-1-AP

    WB result of BCL3 antibody (23959-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL3 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using BCL3 Polyclonal antibody (23959-1-AP)

    WB analysis of HEK-293 using 23959-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BCL3 Polyclonal antibody (23959-1-AP)

    WB analysis of HEK-293 using 23959-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using BCL3 Polyclonal antibody (23959-1-AP)

    WB analysis of A549 using 23959-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 23959-1-AP (BCL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BCL3 Polyclonal antibody (23959-1-AP)

    IP experiment of HEK-293 using 23959-1-AP

    IP result of anti-BCL3 (IP:23959-1-AP, 4ug; Detection:23959-1-AP 1:600) with HEK-293 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of human lymphoma using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of human lymphoma using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of human lymphoma using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of human lymphoma using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of mouse spleen using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of mouse spleen using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23959-1-AP (BCL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of human lymphoma using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BCL3 Polyclonal antibody (23959-1-AP)

    IHC staining of human lymphoma using 23959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23959-1-AP (BCL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCL3 Polyclonal antibody (23959-1-AP)

    IF Staining of HepG2 using 23959-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (23959-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23959-1-AP targets BCL3 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL3 fusion protein Ag21040 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 3
Calculated molecular weight 446 aa, 47 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC064993
Gene symbol BCL3
Gene ID (NCBI) 602
RRIDAB_2879375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).

Protocols

Product Specific Protocols
WB protocol for BCL3 antibody 23959-1-APDownload protocol
IHC protocol for BCL3 antibody 23959-1-APDownload protocol
IF protocol for BCL3 antibody 23959-1-APDownload protocol
IP protocol for BCL3 antibody 23959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
humanIHC

Nucleic Acids Res

SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators.

Authors - Xiaojun Ma
View Article
humanWB,IP

Mol Cancer Ther

The Discovery of a Novel Antimetastatic Bcl3 Inhibitor.

Authors - Jitka Soukupová
View Article
humanWB

Carcinogenesis

Bcl-3 promotes multi-modal tumour cell migration via NF-κB1 mediated regulation of Cdc42.

Authors - Daniel J Turnham
  • KD Validated
View Article
humanWB,chIP

J Biol Chem

The proto-oncogene Bcl3 induces immune checkpoint PD-L1 expression, mediating proliferation of ovarian cancer cells.

Authors - Yue Zou
  • KD Validated
View Article
humanWB

Int J Oncol

BCL‑3 promotes cyclooxygenase‑2/prostaglandin E2 signalling in colorectal cancer.

Authors - Tracey Jane Collard
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB and IF

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
  • Cell Tissue Type: Bone Marrow MSCs
FH

Ewelina (Verified Customer) (07-03-2019)

This is the only antibody for Western Blot, that worked for me for the detection of endogenous BCL3 in human lymphoma cell lines. It works perfectly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human lymphoma cell lines WT and KO