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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL3 Recombinant antibody, PBS Only

BCL3 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242233B11

Cat no : 84882-5-PBS

Synonyms

Proto-oncogene BCL3, D19S37, BCL4, BCL-3, B-cell lymphoma 3 protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BCL3 Recombinant antibody (84882-5-RR)

    IHC staining of mouse spleen using 84882-5-RR (same clone as 84882-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using BCL3 Recombinant antibody (84882-5-RR)

    IHC staining of mouse spleen using 84882-5-RR (same clone as 84882-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCL3 Recombinant antibody (84882-5-RR)

    IF Staining of HeLa using 84882-5-RR (same clone as 84882-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCL3 Recombinant antibody (84882-5-RR)

    IF Staining of HepG2 using 84882-5-RR (same clone as 84882-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84882-5-RR

    Affinity and Kinetic Characterization of 84882-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84882-5-RR against Human BCL3 were performed. The affinity constant is 0.581 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84882-5-PBS targets BCL3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCL3 fusion protein Ag13375 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 3
Calculated molecular weight 446 aa, 47 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC064993
Gene symbol BCL3
Gene ID (NCBI) 602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).