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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL6 Monoclonal antibody, PBS Only

BCL6 Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E6B1

Cat no : 66340-1-PBS

Synonyms

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51

Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using BCL6 Monoclonal antibody (66340-1-Ig)

    WB analysis of Daudi using 66340-1-Ig (same clone as 66340-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using BCL6 Monoclonal antibody (66340-1-Ig)

    WB analysis of Ramos using 66340-1-Ig (same clone as 66340-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using BCL6 Monoclonal antibody (66340-1-Ig)

    WB analysis of Raji using 66340-1-Ig (same clone as 66340-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL6 Monoclonal antibody (66340-1-Ig)

    IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 Antibody) at dilution of 1:1000 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). The signal was enhanced by soaking the slide in 1% CuSO4 for 10 minutes after DAB development. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using BCL6 Monoclonal antibody (66340-1-Ig)

    IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer (pH9).The signal was enhanced by soaking the slide in 1% CuSO4 for 10 minutes after DAB development. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Ramos cells using BCL6 Monoclonal antibody (66340-1-Ig)

    FC experiment of Ramos using 66340-1-Ig (same clone as 66340-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.20 ug/test Anti-Human BCL6 (66340-1-Ig, Clone:1E6B1) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66340-1-PBS targets BCL6 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCL6 fusion protein Ag15519 相同性解析による交差性が予測される生物種
Full Name B-cell CLL/lymphoma 6
Calculated molecular weight 706 aa, 79 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC150184
Gene symbol BCL6
Gene ID (NCBI) 604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages. This antibody only recognize human species.