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KD/KO Validated

BCL7B Polyclonal antibody

Name: BCL7B Polyclonal antibody
Brand: Proteintech
SKU: 11740-1-AP

BCL7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11740-1-AP

Synonyms

B cell CLL/lymphoma 7B

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HeLa using 11740-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11740-1-AP
WB result of BCL7B antibody (11740-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL7B transfected HeLa cells.
WB analysis of human brain using 11740-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 11740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11740-1-AP (BCL7B antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 11740-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11740-1-AP (BCL7B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11740-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

11740-1-AP targets BCL7B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	BCL7B fusion protein Ag2333 相同性解析による交差性が予測される生物種
Full Name	B-cell CLL/lymphoma 7B
Calculated molecular weight	22 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC000956
Gene symbol	BCL7B
Gene ID (NCBI)	9275
RRID	AB_2063596
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for BCL7B antibody 11740-1-AP	Download protocol
IHC protocol for BCL7B antibody 11740-1-AP	Download protocol
IF protocol for BCL7B antibody 11740-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro. Authors - Daowen Li View Article
mouse	WB	Aging (Albany NY) S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway. Authors - Zhenxuan Shao View Article
mouse	WB,IHC	Sci Rep The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function. Authors - Lena Wischhof KO Validated View Article
human	WB,CoIP	Life Sci Alliance BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression Authors - Nicholas Dietrich KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCL7B Polyclonal antibody

BCL7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HeLa using 11740-1-AP

WB analysis of HeLa using 11740-1-AP

WB analysis of human brain using 11740-1-AP

IHC staining of human colon cancer using 11740-1-AP

IHC staining of human colon cancer using 11740-1-AP

IHC staining of human lung cancer using 11740-1-AP

IHC staining of human lung cancer using 11740-1-AP

IHC staining of human pancreas using 11740-1-AP

IHC staining of human pancreas using 11740-1-AP

IHC staining of human colon cancer using 11740-1-AP

IF Staining of HeLa using 11740-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro.

S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway.

The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function.

BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression