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Validation Data Gallery

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  • Western Blot (WB) analysis of SW480 cells using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis of SW480 using 68611-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis using 68611-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis of RAW 264.7 using 68611-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BCOR Monoclonal antibody (68611-1-Ig)

    WB analysis of HEK-293 using 68611-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68611-1-Ig (BCOR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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BCOR Monoclonal antibody

BCOR Monoclonal Antibody for WB, ELISA
Cat No. 68611-1-Ig
Clone No.1F12H6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

ANOP2, BCOR, FLJ20285, FLJ38041, KIAA1575, MAA2, MCOPS2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, SW480 cells, HEK-293 cells, RAW 264.7 cells, MCF-7 cells, Jurkat cells, MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68611-1-Ig targets BCOR in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCOR fusion protein Ag33785 相同性解析による交差性が予測される生物種
Full Name BCL6 co-repressor
Calculated molecular weight 1703 aa, 186 kDa
Observed molecular weight186-200 kDa
GenBank accession numberBC009675
Gene Symbol BCOR
Gene ID (NCBI) 54880
RRIDAB_3085306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6W2J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem.

Protocols

Product Specific Protocols
WB protocol for BCOR antibody 68611-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols