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  • Featured Product
  • KD/KO Validated

BCR Polyclonal antibody, PBS Only

BCR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22585-1-PBS

Synonyms

Renal carcinoma antigen NY-REN-26, EC:2.7.11.1, breakpoint cluster region, BCR1, BCR 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of Y79 using 22585-1-AP (same clone as 22585-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of HEK-293 using 22585-1-AP (same clone as 22585-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of MCF-7 using 22585-1-AP (same clone as 22585-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of HeLa using 22585-1-AP (same clone as 22585-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of K-562 using 22585-1-AP (same clone as 22585-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COS-7 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of COS-7 using 22585-1-AP (same clone as 22585-1-PBS)

    COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BCR Polyclonal antibody (22585-1-AP)

    IP experiment of HEK-293 using 22585-1-AP (same clone as 22585-1-PBS)

    IP result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using BCR Polyclonal antibody (22585-1-AP)

    IHC staining of human skin using 22585-1-AP (same clone as 22585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using BCR Polyclonal antibody (22585-1-AP)

    IHC staining of human skin using 22585-1-AP (same clone as 22585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCR Polyclonal antibody (22585-1-AP)

    IF Staining of HeLa using 22585-1-AP (same clone as 22585-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCR antibody (22585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCR Polyclonal antibody (22585-1-AP)

    IF Staining of HepG2 using 22585-1-AP (same clone as 22585-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22585-1-PBS targets BCR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCR fusion protein Ag18281 相同性解析による交差性が予測される生物種
Full Name breakpoint cluster region
Calculated molecular weight 143 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC066122
Gene symbol BCR
Gene ID (NCBI) 613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.