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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCR Monoclonal antibody

BCR Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2C2E3

Cat no : 67260-1-Ig

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, FLJ16453, PHL

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using BCR Monoclonal antibody (67260-1-Ig)

    WB analysis of Jurkat using 67260-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BCR Monoclonal antibody (67260-1-Ig)

    WB analysis of HEK-293 using 67260-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BCR Monoclonal antibody (67260-1-Ig)

    WB analysis of HeLa using 67260-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67260-1-Ig (BCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K-562 cells using BCR Monoclonal antibody (67260-1-Ig)

    FC experiment of K-562 using 67260-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human BCR (67260-1-Ig, Clone:2C2E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67260-1-Ig targets BCR in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BCR fusion protein Ag28953 相同性解析による交差性が予測される生物種
Full Name breakpoint cluster region
Calculated molecular weight 143 kDa
Observed molecular weight 150-160 kDa
GenBank accession numberBC066122
Gene symbol BCR
Gene ID (NCBI) 613
RRIDAB_2882531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for BCR antibody 67260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols