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BCRP/ABCG2 Polyclonal antibody
BCRP/ABCG2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27286-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, MCF-7 cells, SGC-7901 cells, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 45 publications below |
| IHC | See 11 publications below |
| IF | See 1 publications below |
Product Information
27286-1-AP targets BCRP/ABCG2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BCRP/ABCG2 fusion protein Ag25911 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family G (WHITE), member 2 |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 68-72 kDa |
| GenBank accession number | BC021281 |
| Gene symbol | ABCG2 |
| Gene ID (NCBI) | 9429 |
| RRID | AB_2880830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BCRP/ABCG2 antibody 27286-1-AP | Download protocol |
| IHC protocol for BCRP/ABCG2 antibody 27286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer | ||
Mol Cancer Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer. | ||
PLoS Biol A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance | ||
Cancer Lett Lovastatin/SN38 co-loaded liposomes amplified ICB therapeutic effect via remodeling the immunologically-cold colon tumor and synergized stimulation of cGAS-STING pathway | ||
Mol Ther CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma. | ||
Phytomedicine Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marina (Verified Customer) (07-24-2024) | Incubation with primary antibody ON at 4 C. Incubation with secondary antibody (rabbit) 2h at RT.
|
FH Caitlin (Verified Customer) (07-12-2024) | The antibody worked well in that there was minimal background staining. However, the protein shows as multiple bands. Although, this is possibly due to a protein preparation issue rather than an antibody issue
|
FH Udesh (Verified Customer) (09-12-2022) | Used in Western Blot at 1:3000 Dilution and it detected a band at 75 kDa.
 |
