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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BCRP/ABCG2 Recombinant antibody

BCRP/ABCG2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240959A4

Cat no : 83835-1-RR

Synonyms

ABCG2, BCRP,ABCG2, BCRP, ABCP, ABC15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BCRP,ABCG2 Recombinant antibody (83835-1-RR)

    WB analysis using 83835-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83835-1-RR

    Affinity and Kinetic Characterization of 83835-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, U-251 cells, MCF-7 cells, SGC-7901 cells, HepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83835-1-RR is BCRP/ABCG2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BCRP/ABCG2 fusion protein Ag25911 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family G (WHITE), member 2
Calculated molecular weight 70 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC021281
Gene symbol ABCG2
Gene ID (NCBI) 9429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.

Protocols

Product Specific Protocols
WB protocol for BCRP/ABCG2 antibody 83835-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols