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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BDH2 Polyclonal antibody (27207-1-AP)

    WB analysis using 27207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BDH2 Polyclonal antibody (27207-1-AP)

    WB analysis of HeLa using 27207-1-AP

    WB result of BDH2 antibody (27207-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using BDH2 Polyclonal antibody (27207-1-AP)

    WB analysis using 27207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BDH2 Polyclonal antibody (27207-1-AP)

    WB analysis of HeLa using 27207-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BDH2 Polyclonal antibody (27207-1-AP)

    IHC staining of human breast cancer using 27207-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BDH2 Polyclonal antibody (27207-1-AP)

    IHC staining of human breast cancer using 27207-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

BDH2 Polyclonal antibody

BDH2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27207-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

BDH2, DHRS6, EFA6R, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27207-1-AP targets BDH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BDH2 fusion protein Ag24934 相同性解析による交差性が予測される生物種
Full Name 3-hydroxybutyrate dehydrogenase, type 2
Observed molecular weight 27 kDa
GenBank accession numberBC095414
Gene Symbol BDH2
Gene ID (NCBI) 56898
RRIDAB_2880800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for BDH2 antibody 27207-1-APDownload protocol
IHC protocol for BDH2 antibody 27207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus.

Authors - Xiaofei Gao
View Article
human,mouseWB,IHC

J Hematol Oncol

Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.

Authors - Tianxiao Yang
  • KD Validated
View Article
humanIHC

J Exp Clin Cancer Res

BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.

Authors - Jia-Zhou Liu
View Article