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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BDNF Monoclonal antibody, PBS Only

BDNF Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5C5

Cat no : 66292-1-PBS

Synonyms

Abrineurin, BDNF

Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using BDNF Monoclonal antibody (66292-1-Ig)

    WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using BDNF Monoclonal antibody (66292-1-Ig)

    WB analysis of fetal human brain using 66292-1-Ig (same clone as 66292-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using BDNF Monoclonal antibody (66292-1-Ig)

    IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using BDNF Monoclonal antibody (66292-1-Ig)

    IF Staining of PC-12 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66292-1-PBS targets BDNF in WB, IF, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BDNF fusion protein Ag11329 相同性解析による交差性が予測される生物種
Full Name brain-derived neurotrophic factor
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC029795
Gene symbol BDNF
Gene ID (NCBI) 627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).