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Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using BDNF Monoclonal antibody (66292-1-Ig)

    WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using BDNF Monoclonal antibody (66292-1-Ig)

    WB analysis of fetal human brain using 66292-1-Ig (same clone as 66292-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using BDNF Monoclonal antibody (66292-1-Ig)

    IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using BDNF Monoclonal antibody (66292-1-Ig)

    IF Staining of PC-12 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using BDNF Monoclonal antibody (66292-1-Ig)

    IF Staining of PC-12 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

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BDNF Monoclonal antibody, PBS Only

BDNF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66292-1-PBS
Clone No.1E5C5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66292-1-PBS targets BDNF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BDNF fusion protein Ag11329 相同性解析による交差性が予測される生物種
Full Name brain-derived neurotrophic factor
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
RRIDAB_2881675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23560
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).