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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BEND3 Polyclonal antibody (23101-1-AP)

    WB analysis using 23101-1-AP (same clone as 23101-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23101-1-AP (BEND3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BEND3 Polyclonal antibody (23101-1-AP)

    IHC staining of human liver cancer using 23101-1-AP (same clone as 23101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BEND3 Polyclonal antibody (23101-1-AP)

    IHC staining of human liver cancer using 23101-1-AP (same clone as 23101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23101-1-AP (BEND3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using BEND3 Polyclonal antibody (23101-1-AP)

    IF Staining of HEK-293 using 23101-1-AP (same clone as 23101-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BEND3 antibody (23101-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 23101-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23101-1-PBS targets BEND3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BEND3 fusion protein Ag19137 相同性解析による交差性が予測される生物種
Full Name BEN domain containing 3
Calculated molecular weight 828 aa, 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC131628
Gene Symbol BEND3
Gene ID (NCBI) 57673
RRIDAB_2879210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5T5X7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BEND3 is a quadruple BEN domain-containing protein that associates with heterochromatin and functions as a transcriptional repressor. BEND3 is highly expressed in pluripotent cells, and the induction of differentiation results in the down-regulation of BEND3. (PMID: 35217604, PMID: 25600804)