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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BEND4 Polyclonal antibody

BEND4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24711-1-AP

Synonyms

BEN domain containing 4, BEND4, CCDC4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using BEND4 Polyclonal antibody (24711-1-AP)

    WB analysis of mouse testis using 24711-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24711-1-AP (BEND4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using BEND4 Polyclonal antibody (24711-1-AP)

    IHC staining of human heart using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BEND4 Polyclonal antibody (24711-1-AP)

    IHC staining of human colon cancer using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using BEND4 Polyclonal antibody (24711-1-AP)

    IHC staining of human heart using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BEND4 Polyclonal antibody (24711-1-AP)

    IHC staining of human colon cancer using 24711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24711-1-AP (BEND4 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24711-1-AP is BEND4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BEND4 fusion protein Ag20169 相同性解析による交差性が予測される生物種
Full Name BEN domain containing 4
Calculated molecular weight 534 aa, 58 kDa
Observed molecular weight 55-58 kDa, 33-36 kDa
GenBank accession numberBC128592
Gene symbol BEND4
Gene ID (NCBI) 389206
RRIDAB_2879684
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BEND4, also named as CCDC4, has 5 isoforms with MW 55-58 kDa, 45-48 kDa and 36 kDa.

Protocols

Product Specific Protocols
WB protocol for BEND4 antibody 24711-1-APDownload protocol
IHC protocol for BEND4 antibody 24711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols