Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse colon tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
20443-1-AP targets Bestrophin-3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Bestrophin-3 fusion protein Ag14255 相同性解析による交差性が予測される生物種 |
| Full Name | bestrophin 3 |
| Calculated molecular weight | 668 aa, 76 kDa |
| Observed molecular weight | 76-78 kDa, 50-55 kDa |
| GenBank accession number | BC028087 |
| Gene Symbol | BEST3 |
| Gene ID (NCBI) | 144453 |
| RRID | AB_10666435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N1M1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Bestrophin-3 (BEST3) is a member of the Bestrophin (VMD2) Ca2+-activated Cl channel gene family. Bestrophin-3 is involved in the regulation of cell proliferation, apoptosis and differentiation of a variety of physiological functions (PMID: 25329324). Human BEST3 gene is located at chromosome 12q15. The expression of BEST3 was predominantly observed in skeletal muscle and weaker in brain, spinal cord, bone marrow, retina, thymus, and testis (PMID: 12032738).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Bestrophin-3 antibody 20443-1-AP | Download protocol |
| IHC protocol for Bestrophin-3 antibody 20443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum. | ||
J Mol Cell Cardiol Sarcolemmal Ca(2+)-entry through L-type Ca(2+) channels controls the profile of Ca(2+)-activated Cl(-) current in canine ventricular myocytes. |