• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

Biglycan Polyclonal antibody

Biglycan Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 16409-1-AP

Synonyms

BGN, PRG1, PG-S1, PGI, PG S1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Biglycan Polyclonal antibody (16409-1-AP)

    WB analysis using 16409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Biglycan Polyclonal antibody (16409-1-AP)

    WB analysis of mouse skeletal muscle using 16409-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cartilage tissue using Biglycan Polyclonal antibody (16409-1-AP)

    WB analysis of pig cartilage using 16409-1-AP

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 16409-1-AP (Biglycan Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Biglycan Polyclonal antibody (16409-1-AP)

    WB analysis of HeLa using 16409-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Biglycan Polyclonal antibody (16409-1-AP)

    WB analysis of HepG2 using 16409-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP (BGN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human normal colon using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human normal colon using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16409-1-AP (Biglycan antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human lung cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human colon cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human kidney using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human stomach cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human stomach cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human liver using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human liver using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16409-1-AP (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human stomach cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human lung using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human lung using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human liver cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human liver cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human liver cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human liver cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 16409-1-AP (BGN antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human renal cell carcinoma using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human renal cell carcinoma using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of mouse lung using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of mouse lung using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Biglycan Polyclonal antibody (16409-1-AP)

    IHC staining of human stomach cancer using 16409-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16409-1-AP (BGN antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Biglycan Polyclonal antibody (16409-1-AP)

    IF Staining of HepG2 using 16409-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Biglycan antibody (16409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HepG2 cells, mouse skeletal muscle tissue, pig cartilage tissue
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human kidney tissue, human liver tissue, human liver cancer tissue, human lung tissue, human renal cell carcinoma tissue, human stomach cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16409-1-AP targets Biglycan in WB, IHC, IF/ICC, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Biglycan fusion protein Ag0683 相同性解析による交差性が予測される生物種
Full Name biglycan
Calculated molecular weight 42 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC004244
Gene symbol Biglycan
Gene ID (NCBI) 633
RRIDAB_10804656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).

Protocols

Product Specific Protocols
WB protocol for Biglycan antibody 16409-1-APDownload protocol
IHC protocol for Biglycan antibody 16409-1-APDownload protocol
IF protocol for Biglycan antibody 16409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncoimmunology

Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.

Authors - Shaoquan Zheng
View Article
humanWB,IF

Cancers (Basel)

Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.

Authors - Kanakaraju Manupati
  • KD Validated
View Article
mouseIHC

J Biol Eng

Proteomic analysis of decellularized mice liver and kidney extracellular matrices

Authors - Anna-Maria Diedrich
View Article
humanWB,IHC

Oncoimmunology

Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.

Authors - Karthikeyan Subbarayan
View Article
ratIF

Cell Prolif

Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.

Authors - Yunjiao Wang
View Article
humanIHC

Cancer Med

Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma

Authors - Wanyi Xiao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Federica (Verified Customer) (12-08-2023)

Leica Bond Rxm Red Chronogenic Kit Antigen Retrival ER1 (ph6) for 30 min

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Placenta healthy
Biglycan Antibody Immunohistochemistry validation (1:500 dilution) in Placenta healthy (Cat no:16409-1-AP)