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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIST1 Polyclonal antibody

MIST1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25140-1-AP

Synonyms

BHLHA15, BHLHB8, MIST 1, MIST1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using MIST1 Polyclonal antibody (25140-1-AP)

    WB analysis of mouse liver using 25140-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using MIST1 Polyclonal antibody (25140-1-AP)

    WB analysis of rat pancreas using 25140-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using MIST1 Polyclonal antibody (25140-1-AP)

    WB analysis of rat pancreas using 25140-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 25140-1-AP (MIST1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using MIST1 Polyclonal antibody (25140-1-AP)

    IP experiment of mouse liver using 25140-1-AP

    IP result of anti-MIST1 (IP:25140-1-AP, 3ug; Detection:25140-1-AP 1:300) with mouse liver tissue lysate 6800ug.

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Tested Applications

Positive WB detected inmouse liver tissue, rat pancreas tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25140-1-AP is MIST1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIST1 fusion protein Ag18199 相同性解析による交差性が予測される生物種
Full Name basic helix-loop-helix family, member a15
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC113394
Gene symbol MIST1
Gene ID (NCBI) 168620
RRIDAB_2879920
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MIST1 antibody 25140-1-APDownload protocol
IP protocol for MIST1 antibody 25140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols