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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BHLHE40 Polyclonal antibody (17895-1-AP)

    WB analysis using 17895-1-AP (same clone as 17895-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17895-1-AP (BHLHE40 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using BHLHE40 Polyclonal antibody (17895-1-AP)

    IP experiment of HeLa using 17895-1-AP (same clone as 17895-1-PBS)

    IP result of anti-BHLHE40 (IP:17895-1-AP, 4ug; Detection:17895-1-AP 1:1000) with HeLa cells lysate 1800 ug. This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using BHLHE40 Polyclonal antibody (17895-1-AP)

    IHC staining of mouse stomach using 17895-1-AP (same clone as 17895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using BHLHE40 Polyclonal antibody (17895-1-AP)

    IHC staining of human endometrial cancer using 17895-1-AP (same clone as 17895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using BHLHE40 Polyclonal antibody (17895-1-AP)

    IHC staining of human endometrial cancer using 17895-1-AP (same clone as 17895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using BHLHE40 Polyclonal antibody (17895-1-AP)

    IHC staining of mouse stomach using 17895-1-AP (same clone as 17895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BHLHE40 Polyclonal antibody (17895-1-AP)

    IHC staining of human pancreas cancer using 17895-1-AP (same clone as 17895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BHLHE40 Polyclonal antibody (17895-1-AP)

    IHC staining of human pancreas cancer using 17895-1-AP (same clone as 17895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17895-1-AP (BHLHE40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17895-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17895-1-PBS targets BHLHE40 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BHLHE40 fusion protein Ag12236 相同性解析による交差性が予測される生物種
Full Name basic helix-loop-helix family, member e40
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC082238
Gene Symbol BHLHE40
Gene ID (NCBI) 8553
RRIDAB_2065351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14503
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BHLHE40 (Basic Helix-Loop-Helix Family Member E40), also known as BHLHB2, STRA13, DEC1, or SHARP2, is a member of the basic helix-loop-helix (bHLH) protein family, a large superfamily of transcriptional regulators expressed in many organisms. BHLHE40 is known to regulate a wide variety of essential cellular processes, including cell cycle, cellular proliferation, programmed cell death, cellular development and differentiation, as well as circadian rhythms (PMID: 34551158). It is reported that BHLHE40 is overexpressed in gastric, breast, and brain tumors; and downregulated in colorectal, esophageal, pancreatic and lung cancer (PMID: 32577154).