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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BHLHE40 Monoclonal antibody

BHLHE40 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A3E1

Cat no : 68435-1-Ig

Synonyms

BHLHB2, BHLHE40, DEC1, FLJ99214, SHARP 2, SHARP2, STRA13, Stra14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BHLHE40 Monoclonal antibody (68435-1-Ig)

    WB analysis using 68435-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68435-1-Ig (BHLHE40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCalu-3 cells, A549 cells, HCT 116 cells, MDA-MB-468 cells, Saos-2 cells, HeLa cells, PC-3 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68435-1-Ig targets BHLHE40 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BHLHE40 fusion protein Ag12236 相同性解析による交差性が予測される生物種
Full Name basic helix-loop-helix family, member e40
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC082238
Gene symbol BHLHE40
Gene ID (NCBI) 8553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BHLHE40 (Basic Helix-Loop-Helix Family Member E40), also known as BHLHB2, STRA13, DEC1, or SHARP2, is a member of the basic helix-loop-helix (bHLH) protein family, a large superfamily of transcriptional regulators expressed in many organisms. BHLHE40 is known to regulate a wide variety of essential cellular processes, including cell cycle, cellular proliferation, programmed cell death, cellular development and differentiation, as well as circadian rhythms (PMID: 34551158). It is reported that BHLHE40 is overexpressed in gastric, breast, and brain tumors; and downregulated in colorectal, esophageal, pancreatic and lung cancer (PMID: 32577154).

Protocols

Product Specific Protocols
WB protocol for BHLHE40 antibody 68435-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols