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  • Featured Product
  • KD/KO Validated

BID Monoclonal antibody, PBS Only

BID Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2E5

Cat no : 60301-1-PBS

Synonyms

BID, FP497, p11 BID, p22 BID

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BID Monoclonal antibody (60301-1-Ig)

    WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    WB result of BID antibody (60301-1-Ig, 1:8000) with si-Control and si-BID transfected HeLa cells. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BID Monoclonal antibody (60301-1-Ig)

    WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using BID Monoclonal antibody (60301-1-Ig)

    WB analysis of Jurkat using 60301-1-Ig (same clone as 60301-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BID Monoclonal antibody (60301-1-Ig)

    IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BID Monoclonal antibody (60301-1-Ig)

    IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BID Monoclonal antibody (60301-1-Ig)

    IF Staining of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60301-1-Ig (BID antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60301-1-PBS targets BID in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BID fusion protein Ag21005 相同性解析による交差性が予測される生物種
Full Name BH3 interacting domain death agonist
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009197
Gene symbol BID
Gene ID (NCBI) 637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).