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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BID Recombinant antibody (84944-4-RR)

    WB analysis using 84944-4-RR (same clone as 84944-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84944-4-RR (BID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using BID Recombinant antibody (84944-4-RR)

    FC experiment of HeLa using 84944-4-RR (same clone as 84944-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using BID Recombinant antibody (84944-4-RR)

    FC experiment of MCF-7 using 84944-4-RR (same clone as 84944-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

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BID Recombinant antibody, PBS Only

BID Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84944-4-PBS
Clone No.242460F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

p22 BID, BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84944-4-PBS targets BID in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name BH3 interacting domain death agonist
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55957
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)