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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BIN3 Polyclonal antibody, PBS Only

BIN3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11215-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using BIN3 Polyclonal antibody (11215-1-AP)

    WB analysis of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BIN3 Polyclonal antibody (11215-1-AP)

    WB analysis of HeLa using 11215-1-AP (same clone as 11215-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using BIN3 Polyclonal antibody (11215-1-AP)

    IP experiment of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

    IP result of anti-BIN3 (IP:11215-1-AP, 4ug; Detection:11215-1-AP 1:300) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BIN3 Polyclonal antibody (11215-1-AP)

    IHC staining of human prostate cancer using 11215-1-AP (same clone as 11215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11215-1-AP (BIN3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11215-1-PBS targets BIN3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BIN3 fusion protein Ag1713 相同性解析による交差性が予測される生物種
Full Name bridging integrator 3
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC009824
Gene symbol BIN3
Gene ID (NCBI) 55909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).