cIAP2 Polyclonal antibody

Name: cIAP2 Polyclonal antibody
Brand: Proteintech
SKU: 24304-1-AP

cIAP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24304-1-AP

Synonyms

BIRC3, CIAP 2, Cellular inhibitor of apoptosis 2, C IAP2, BIRC 3

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 24304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 24304-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24304-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 24304-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 24304-1-AP (cIAP2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Daudi using 24304-1-AP
IP result of anti-cIAP2 (IP:24304-1-AP, 4ug; Detection:24304-1-AP 1:1000) with Daudi cells lysate 2800ug.
IHC staining of human tonsillitis using 24304-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 24304-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24304-1-AP (cIAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 24304-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 24304-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 24304-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 24304-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24304-1-AP (cIAP2 antibody at dilution of 1:50 (under 40x lens).

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Cat No. 24304-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Daudi cells, HeLa cells, RAW 264.7 cells, U-937 cells, Raji cells
Positive IP detected in	Daudi cells
Positive IHC detected in	human tonsillitis tissue, human lung cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 17 publications below
IHC	See 5 publications below

Product Information

24304-1-AP targets cIAP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	cIAP2 fusion protein Ag20143 相同性解析による交差性が予測される生物種
Full Name	baculoviral IAP repeat-containing 3
Calculated molecular weight	604 aa, 68 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC027485
Gene symbol	cIAP2
Gene ID (NCBI)	330
RRID	AB_2879485
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.

Protocols

Product Specific Protocols
WB protocol for cIAP2 antibody 24304-1-AP	Download protocol
IHC protocol for cIAP2 antibody 24304-1-AP	Download protocol
IP protocol for cIAP2 antibody 24304-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform. Authors - Maria Zachari View Article
human	WB	Oncotarget The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis. Authors - Tian-Hao Liu View Article
human	IHC	Front Oncol Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer. Authors - Jinguo Zhang View Article
human,mouse	WB	Biomed Pharmacother Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo. Authors - Lei Wang View Article
human	IHC	Cancer Med Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21. Authors - Shu-Mei Chen View Article
human	WB	Cancer Biol Ther Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly. Authors - Liu Qin Q View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

cIAP2 Polyclonal antibody

cIAP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 24304-1-AP

WB analysis of RAW 264.7 using 24304-1-AP

WB analysis of HeLa using 24304-1-AP

WB analysis of U-937 using 24304-1-AP

IP experiment of Daudi using 24304-1-AP

IHC staining of human tonsillitis using 24304-1-AP

IHC staining of human tonsillitis using 24304-1-AP

IHC staining of human lung cancer using 24304-1-AP

IHC staining of human lung cancer using 24304-1-AP

IHC staining of human testis using 24304-1-AP

IHC staining of human testis using 24304-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.

The putative tumor activator ARHGEF3 promotes nasopharyngeal carcinoma cell pathogenesis by inhibiting cellular apoptosis.

Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.

Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.

Targeting inhibitors of apoptosis proteins suppresses medulloblastoma cell proliferation via G2/M phase arrest and attenuated neddylation of p21.

Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly.