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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BLK Polyclonal antibody

BLK Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10510-1-AP

Synonyms

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK, p55 Blk, Tyrosine protein kinase Blk

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using BLK Polyclonal antibody (10510-1-AP)

    WB analysis of Jurkat using 10510-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using BLK Polyclonal antibody (10510-1-AP)

    WB analysis of Raji using 10510-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using BLK Polyclonal antibody (10510-1-AP)

    WB analysis of SH-SY5Y using 10510-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using BLK Polyclonal antibody (10510-1-AP)

    IP experiment of SH-SY5Y using 10510-1-AP

    IP result of anti-BLK (IP:10510-1-AP, 4ug; Detection:10510-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BLK Polyclonal antibody (10510-1-AP)

    IHC staining of human pancreas cancer using 10510-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using BLK Polyclonal antibody (10510-1-AP)

    IHC staining of human pancreas cancer using 10510-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, Raji cells, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10510-1-AP targets BLK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLK fusion protein Ag0795 相同性解析による交差性が予測される生物種
Full Name B lymphoid tyrosine kinase
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC007371
Gene symbol BLK
Gene ID (NCBI) 640
RRIDAB_2274754
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

Protocols

Product Specific Protocols
WB protocol for BLK antibody 10510-1-APDownload protocol
IHC protocol for BLK antibody 10510-1-APDownload protocol
IP protocol for BLK antibody 10510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Signal Transduct Target Ther

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin

Authors - Cefan Zhou
View Article
humanWB

Cancer Cell

Molecular targets of glucocorticoids that elucidate their therapeutic efficacy in aggressive lymphomas

Authors - Jaewoo Choi
View Article