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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BLM Polyclonal antibody (30254-1-AP)

    WB analysis using 30254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BLM Polyclonal antibody (30254-1-AP)

    WB analysis using 30254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using BLM Polyclonal antibody (30254-1-AP)

    WB analysis using 30254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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BLM Polyclonal antibody

BLM Polyclonal Antibody for WB, ELISA
Cat No. 30254-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

RECQL3, RECQL2, RECQ2, BS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293T cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30254-1-AP targets BLM in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLM fusion protein Ag32996 相同性解析による交差性が予測される生物種
Full Name Bloom syndrome, RecQ helicase-like
Calculated molecular weight159 kDa
Observed molecular weight160-175 kDa
Gene Symbol BLM
Gene ID (NCBI) 641
RRIDAB_3086282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLM participates in DNA replication and repair, it is involved in 5'-end resection of DNA during double-strand break (DSB) repair. BLM can also be recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.

Protocols

Product Specific Protocols
WB protocol for BLM antibody 30254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

YY1 is involved in homologous recombination inhibition at guanine quadruplex sites in human cells

Authors - Xinyu Cui
View Article