Validation Data Gallery
Tested Applications
| Positive WB detected in | human colon tissue, mouse pancreas tissue, mouse spleen tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14941-1-AP targets BLMH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BLMH fusion protein Ag6888 相同性解析による交差性が予測される生物種 |
| Full Name | bleomycin hydrolase |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC003616 |
| Gene Symbol | BLMH |
| Gene ID (NCBI) | 642 |
| RRID | AB_2064803 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13867 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BLMH antibody 14941-1-AP | Download protocol |
| IHC protocol for BLMH antibody 14941-1-AP | Download protocol |
| IF protocol for BLMH antibody 14941-1-AP | Download protocol |
| IP protocol for BLMH antibody 14941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Mol Biol DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts. | ||
Int J Oncol N6-methyladenosine-induced long non-coding RNA PVT1 regulates the miR-27b-3p/BLM axis to promote prostate cancer progression | ||
J Dermatol Sci Increased production of natural moisturizing factors and bleomycin hydrolase activity in elderly human skin | ||
Cancer Gene Ther METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1 |