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Species Reactivity

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  • Featured Product
  • KD/KO Validated

BLVRA Polyclonal antibody

BLVRA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10775-1-AP

Synonyms

BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BLVRA Polyclonal antibody (10775-1-AP)

    WB analysis using 10775-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using BLVRA Polyclonal antibody (10775-1-AP)

    WB analysis of L02 using 10775-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using BLVRA Polyclonal antibody (10775-1-AP)

    WB analysis of mouse liver using 10775-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human prostate cancer using 10775-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human pancreas using 10775-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human pancreas using 10775-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human prostate cancer using 10775-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BLVRA Polyclonal antibody (10775-1-AP)

    IHC staining of human prostate cancer using 10775-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using BLVRA Polyclonal antibody (10775-1-AP)

    IF Staining of L02 using 10775-1-AP

    Immunofluorescent analysis of L02 cells using 10775-1-AP (BLVRA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, L02 cells, mouse liver tissue, HepG2 cells, HuH-7 cells, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10775-1-AP targets BLVRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BLVRA fusion protein Ag1166 相同性解析による交差性が予測される生物種
Full Name biliverdin reductase A
Calculated molecular weight33.2 kDa
Observed molecular weight 37-40 kDa, 66 kDa
GenBank accession numberBC008456
Gene symbol BLVRA
Gene ID (NCBI) 644
RRIDAB_2065073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BLVRA(Biliverdin reductase A) is also named as BLVR, BVR and belongs to the biliverdin reductase subfamily.It catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH.This protein can exsit as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

Protocols

Product Specific Protocols
WB protocol for BLVRA antibody 10775-1-APDownload protocol
IHC protocol for BLVRA antibody 10775-1-APDownload protocol
IF protocol for BLVRA antibody 10775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Manag Res

Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway.

Authors - Haiyan Mao
  • KD Validated
View Article
humanIF

Arch Biochem Biophys

Biliverdin reductase deficiency triggers an endothelial-to-mesenchymal transition in human endothelial cells.

Authors - Damian Klóska
View Article
humanWB

EXCLI J

Induction of cell apoptosis by biliverdin reductase inhibitor in MCF-7 and MDA-MB-468 breast cancer cell lines: Experimental and in silico studies.

Authors - Seyedeh Zahra Shahrokhi
View Article