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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

BLVRA Monoclonal antibody, PBS Only

BLVRA Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10F2

Cat no : 68175-1-PBS

Synonyms

BVR A, BVR, BLVR, Biliverdin-IX alpha-reductase, biliverdin reductase A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BLVRA Monoclonal antibody (68175-1-Ig)

    WB analysis using 68175-1-Ig (same clone as 68175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68175-1-Ig (BLVRA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BLVRA Monoclonal antibody (68175-1-Ig)

    IF Staining of A549 using 68175-1-Ig (same clone as 68175-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using BLVRA antibody (68175-1-Ig, Clone: 2A10F2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68175-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68175-1-PBS targets BLVRA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BLVRA fusion protein Ag32730 相同性解析による交差性が予測される生物種
Full Name biliverdin reductase A
Calculated molecular weight33.2 kDa
Observed molecular weight 37-40 kDa, 66 kDa
GenBank accession numberBC008456
Gene symbol BLVRA
Gene ID (NCBI) 644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

BLVRA(Biliverdin reductase A) is also named BLVR, BVR, and belongs to the biliverdin reductase subfamily. It catalyzes the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. This protein can exist as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).